Browsing by Author "Sayeed, Mohammed Aktar"
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Item Antidiarrheal, cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves(Published by www.bsmiab.org, 2020-01-19) Sayeed, Mohammed AktarABSTRACT: The study reports the in vivo antidiarrheal and in vitro cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves (MEHCL). The antidiarrheal activity was evaluated by castor oil-induced diarrhea, whereas the intestinal motility by charcoal marker. In addition, brine shrimp lethality bioassay and human blood clot lysis were used to evaluate the cytotoxic and thrombolytic activities, respectively. In antidiarrheal study, castor oil-induced diarrhea and gastrointestinal motility exhibited a significant dose dependent reduction in diarrhea and defecation and an extremely significant (P < 0.0001) inhibition in intestinal motility and peristalsis index by 200 and 400 mg/kg of MEHCL. The brine shrimp lethality bioassay revealed a considerable cytotoxic effect of MEHCL (LC50= 81.59 μg/mL; R² = 0.927) while in thrombolytic a significant percentage of clot lysis (17.36%, P < 0.01) demonstrated. The findings suggest that H. coccineum leaves could be potential sources for biological activity.Item Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae): In vivo and in vitro approach(www.bsmiab.org, 2020-06-14) Rudra, Sajib; Sawon, Solaman Uddin; Emon, Nazim Uddin; Alam, Safaet; Tareq, Syed Mohammed; Islam, Mohammad Nazmul; Uddin, Md. Rokib; Md Sazid, Abdullah; Hasbe, Abu Nazer; Shakil, Mohammad; Sakib, Shahenur Alam; Sayeed, Mohammed AktarTetrastigma leucostaphylum (Family: Vitaceae) is popular for its medicinal value in Bangladeshi tribal communities. This study aims to investigate several pharmacological values of methanol extract of T. leucostaphylum (METL). In vivo analgesic and anti-inflammatory researches have been implemented by using acetic acid-induced writhing and formalin-induced paw licking test protocols in mice. Furthermore, in vitro thrombolytic and anthelmintic studies have been performed by following the blood clot lysis method and nematode mortality measurement method. In the in vivo study, METL did not minimize the acetic acid-induced writhes prominently but significantly attenuate both the peripheral and inflammatory pain in mice in a dose-dependent manner. In early and late phase, METL 400 (mg/kg, b.w; p.o) showed 39.63 % and 48.73 % paw licking inhibition. Again, METL (100 μL) reflected 56.62 % clot lysis in thrombolytic research. Besides, METL causes the death of nematodes in a dose-dependent manner. The bioassay of the methanol extract of T. leucostaphylum justified the analgesic, anti-inflammatory, thrombolytic and anthelmintic activities of the crude extract and finally suggests the test extract as a wellspring of anti-inflammatory, thrombolytic and anthelmintic agents as a crude drug source.Item Chemical Profiling, Pharmacological Insights and In Silico Studies of Methanol Seed Extract of Sterculia foetida(https://www.mdpi.com/journal/plants, 2021-06-03) Alam, Najmul; Banu, Naureen; Aziz, Arfin Ibn; Barua, Niloy; Ruman, Umme; Jahan, Israt; Jahan, Farhana; Denath, Susmita; Paul, Arkajyoti; Uddin, Nazim; Sayeed, Mohammed Aktar; Emran, Talha Bin; Gandara, Jesus SimalSterculia foetida, also known as jangli badam in Bangladesh, is a traditionally used plant that has pharmacological activities. A qualitative phytochemical analysis was performed to assess the metabolites in a methanolic extract of S. foetida seeds (MESF), and the cytotoxic, thrombolytic, anti-arthritics, analgesic, and antipyretic activities were examined using in vitro, in vivo, and in silico experiments. Quantitative studies were performed through gas chromatography-mass spectroscopy (GC-MS) analysis. The brine shrimp lethality bioassays and clot lysis were performed to investigate the cytotoxic and thrombolytic activities, respectively. The anti-arthritics activity was assessed using the albumin denaturation assay. Analgesic activity was determined using the acetic acidinduced writhing test and the formalin-induced paw-licking test. A molecular docking study was performed, and an online tool was used to perform ADME/T (absorption, distribution, metabolism, and excretion/toxicity) and PASS (Prediction of Activity Spectra for Substances). GC-MS analysis identified 29 compounds in MESF, consisting primarily of phenols, terpenoids, esters, and other organic compounds. MESF showed moderate cytotoxic activity against brine shrimp and significant thrombolytic and anti-arthritics activities compared with the relative standards. The extract also showed a dose-dependent and significant analgesic and antipyretic activities. Docking studies showed that 1-azuleneethanol, acetate returned the best scores for the tested enzymes. These findings suggested that MESF represents a potent source of thrombolytic, anti-arthritic, analgesic, antipyretic agents with moderate cytotoxic effects.Item Chemical, biological and protein-receptor binding profiling of Bauhinia scandens L. stems provide new insights into the management of pain, inflammation, pyrexia and thrombosis((http://creativecommons.org/licenses/by-nc-nd/4.0/)., 2021-09-08) Emon, Nazim Uddin; Rudra, Sajib; Alam, Safaet; Al Haidar, Ibrahim Khalil; Paul, Susmita; Richi, Fahmida Tasnim; Shahriar, Saimon; Sayeed, Mohammed Aktar; Tumpa, Nadia Islam; Ganguly, AmlanBauhinia scandens L. (Family, Fabaceae) is a medicinal plant used for conventional and societal medication in Ayurveda. The present study has been conducted to screen the chemical, pharmacological and biochemical potentiality of the methanol extracts of B. scandens stems (MEBS) along with its related fractions including carbon tetrachloride (CTBS), di-chloromethane (DMBS) and n-butanol (BTBS). UPLC-QTOF–MS has been implemented to analyze the chemical compounds of the methanol extracts of Bauhinia scandens stems. Additionally, antinociceptive and anti-inflammatory effects were performed by following the acetic acid-induced writhing test and formalin-mediated paw licking test in the mice model. The antipyretic investigation was performed by Brewer Yeast induced pyrexia method. The clot lysis method was implemented to screen the thrombolytic activity in human serum. Besides, the in silico study was performed for the five selected chemical compounds of Bauhinia scandens, found by UPLC-QTOF–MS By using Discover Studio 2020, UCSF Chimera, PyRx autodock vina and online tools. The MEBS and its fractions exhibited remarkable inhibition in dose dependant manner in the antinociceptive and antiinflammatory investigations. The antipyretic results of MEBS and DMBS were close to the standard drug indomethacin. Investigation of the thrombolytic effect of MEBS, CTBS, DMBS, and BTBS revealed notable clot-lytic potentials. Besides, the phenolic compounds of the plant extracts revealed strong binding affinity to the COX-1, COX-2, mPGES-1 and plasminogen activator enzymes. To recapitulate, based on the research work, Bauhinia scandens L. stem and its phytochemicals can be considered as prospective wellsprings for novel drug development and discovery by future researchers.Item In vitro Study on Interaction of Ketotifen Fumerate with Paracetamol(CRP, International Islamic University Chittagong, Bangladesh, 2010-12) Sayeed, Mohammed Aktar; Rana, SohelInteraction of ketotifen fumerate and paracetamol was studied in aqueous media. The ability of interaction of ketotifen with paracetamol is dependent on pH of the solution and it has been found that ketotifen forms 1:1 complexes at different pH. The stability constants have been calculated from the Ardon’s spectrophotomeric measurements of the reaction systems. When ketotifen was interacted with paracetamol and the absorbance was determined at 300 nm the stability constants were found of -7.32 and -7.84 at pH 1.6 and 7.4 respectively.Item In vitro-In vivo Studies on Drug Interaction Between Ketotifen and Commonly Prescribed Drugs(International Islamic University Chittagong, 2014-06) Sayeed, Mohammed AktarThe objectives of the present study were to investigate the drug-drug interactions between ketotifen fumarate and some commonly prescribed drugs. Their interactions were identified and confirmed by UV, IR, DSC and HPLC followed by TLC. The in vitro results were correlated with in vivo model to see whether the desired drug concentration could attain into the blood stream or not. Finally attempts have been taken to find out the effects of these complexes on the liver and kidney. Each of the drugs absorption was analyzed in the UV-VIS region. The spectra of pure drugs as well as their 1:1, 1:2 and 2:1 mixtures of ketotifen & paracetamol, ketotifen & domperidone, ketotifen & desloratidine, ketotifen & amoxicillin, ketotifen & metformin, ketotifen & chlorpheniramine, ketotifen & theophylline, ketotifen & salbutamol and ketotifen & diclophenac sodium were studied at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8 and 7.4. At both gastric and intestinal pHs, a sharp breakdown was observed in the curves. Similarly when ketotifen was mixed with metformin, a sharp change was observed in the curve at pHs 0.4, 1.2, 2.8 & 7.4 which indicated drug-drug interactions, whereas the absence of such particular breakdown in the curve of ketotifen and metformin mixture at pHs 2.0, 6.0 and 6.8 revealed the absence of drug interactions. Again when various concentrations comprising 1x10 -5 M to 9x10 -5 M of ketotifen were interacted with chlorpheniramine, sharp changes in the curve were observed at pHs 0.4, 6.0 and 6.8, which demonstrated the presence of drug-drug interactions. On the other hand, the absence of breakdown in the curve of ketotifen and chlorpheniramine mixture at pHs 1.2, 2.0, 2.8 and 7.4 revealed the absence of drug interactions. The stability constant values (k = 1x10 -2 ) for the particular interaction was determined by graphical representation of Ardon’s plot. The stability constants of ketotifen & paracetamol (5.67, 6.36, 7.21, 14.84, 17.97, 38.35, 24.79 at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively), ketotifen & domperidone (77.89, 84.27, 10.01, 28.95, 60.23, 21.85, 114.82 at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively), ketotifen & desloratidine (14.54, 14.07, 16.73, 5.56, 17.49, 5.16, 3.06 at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively), ketotifen & theophylline (6.8, 7.4, 3.5, 4.8, 9.6, 5.7, 18.4 at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively) and ketotifen & amoxicillin (19.8, 13.7, 2.30, 35.7, 14.1, 8.3, 51.6 at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively) were found to be relatively higher at gastric and intestinal pHs. This reflects that there might be relatively stronger complex formation due to interaction between the mentioned drugs. But relatively low stability constant values were seen when the interaction occurred between ketotifen & chlorpheniramine (0.7256, 0.2895, 1.0683, 1.6807, 1.6827, 0.0507, 0.2834 at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively), ketotifen & diclofenac (0.068, 0.057 at pHs 6.8, 7.4 respectively) and ketotifen & metformin (0.03, 0.55, 0.05, 0.01, 0.09, 0.01, 0.77) at pHs 0.4, 1.2, 2.0, 2.8, 6.0, 6.8, 7.4 respectively). If the formation constant is reasonably favorable, two straight lines of different slopes that intersect at a mole ratio corresponding to the mixing ratio in the complex are obtained. In the IR study, the possible interaction between ketotifen fumarate and amoxicillin trihydrate showed characteristic peaks. The peaks of C-Cl (696.33 cm -1 ) was shifted to higher wave number at 703.08 cm -1 and C-O-C group at 1082.11 cm -1 was shifted to higher wave number in the complex at 1099 cm -1 (C-O-C stretching); The peaks of alkenes at 3040 cm -1 was shifted to 3056 cm -1 (=CH). Simultaneously the peaks of isocyanates (-N=C=O) at 2270 cm -1 and carboxylic 3336.99 cm -1 (O-H) were shifted to lower wave numbers in the complex at 2263cm -1 and 3327 cm -1 . On the other hand the interaction between ketotifen fumarate and diclofenac sodium showed that the peaks of acid phosphines at 839.07 cm -1 (P-H stretching) was shifted to higher wave number in the complex at 854.5 cm -1 , the peaks of alkyl halides of 1386.88 cm -1 , amides of 1575.91 cm -1 , amides of 1652.1 cm -1 aromatic group having wave number of 2970.5 cm -1 were showed characteristic peaks in the complexes at 1354.09 cm -1 , 1587.48 cm -1 , 1647.28 cm -1 and 2957 cm -1 respectively. Similarly in case of ketotifen & salbutamol interactions showed characteristic peaks at the peaks of C-H (aromatic group), C-O-C (ether group), C-C (ketone) and O-H (carboxylic acid group) at 838.11 cm -1 , 1085 cm -1 , 1201.7 cm -1 , 2925.17 cm -1 were shifted to higher wave numbers in the complexes at 855.47 cm -1 (C-H stretching), 1099.47 cm -1 (C-O-C stretching), 1224.85 cm -1 (C-C stretching) and 2937.71 cm -1 (O-H). Similarly the interaction between ketotifen and theophylline showed that the peaks of phosphine oxides group at 1180 cm -1 (P=O stretching) and alkenes group at 1378 cm -1 (C-H) were shifted to lower wave numbers in the complex at 1151 cm -1 (P=O stretching) and 1362.77 cm -1 (C-H stretching). On the other hand, peak of amides of 1559.51 cm -1 (N-H stretching) was shifted to higher wave number in the complex at 1588.19 cm -1 (N-H). In the DSC study (ketotifen, domperidone and ketotifen & domperidone mixture) ketotifen exhibited sharp endothermic peak at 193.08 0 C. But the ketotifen-domperidone complex exhibited a sharp new peak at 120.02 0 C (-1.74 mW/mg). On the other hand, DSC of the samples (ketotifen, metformin and ketotifen & metformin mixture) were performed and observed that ketotifen-metformin complex exhibited a sharp new peak at 123.04 0 C (-2.61 mW/mg). But the mixture of ketotifen and theophylline exhibited no sharp peak. The R f values of ketotifen (0.44) and domperidone (0.53) was found to be completely different from ketotifen-domperidone mixture (0.38). The R f values of ketotifen (0.49) and metformin (0.51) was found to be completely different from ketotifen-metformin mixture (0.39) which conclude the stability of the complex for both mixtures. However in case of HPLC the complex which was formed between ketotifen & theophylline and ketotifen & metformin could have been partly dissociated in aqueous medium used to dissolve the sample for HPLC analysis. The multiple comparison table shows that there is a significant difference in absorbances at various mentioned times (30 minutes, 60 minutes, 120 minutes & 180 minutes) to complete the drug interaction between the group that took the single drug as well as mixtures (ketotifen & metformin, ketotifen & paracetamol, ketotifen & salbutamol, ketotifen & amoxicillin, ketotifen & diclofenac) The results were expressed as mean ± SEM values. A probability value less than 0.05 (p < 0.05) was defined to be significant. The results of investigation of hepatotoxicity of combination drug therapy were compared with single drug sample ketotifen. But the groups which receive the combination drug samples ketotifen & metformin (67.5 + 1.44 IU/L) and ketotifen & theophylline (68.5 + 2.5 IU/L) showed a significant increase in SGPOT, and showed a significant decrease of ATPN levels in ketotifen & theophylline mixture (7.0 + 0.07 IU/L) and in ketotifen & metformin mixture (6.13 + 0.73 IU/L). The creatinine concentration was found to be 1.4 mg/dl in case of normal control but it was raised to 3.6 mg/dl when mixture of ketotifen and theophylline was administered. Now we can conclude that the patients having motion sickness and patients who had been suffering from diabetes should take a precaution during coadmintration of ketotifen fumarate & domperidone and ketotifen fumarate & metformin hydrochloride..Item Investigation of the Pharmacological Properties of Lepidagathis hyalina Nees through Experimental Approaches(https://www.mdpi.com/journal/life, 2021-02-25) Fahad, Fowzul Islam; Barua, Niloy; Islam, Md. Shafiqul; Sayem *, Al Jawad; Barua, Koushik; Uddin, Mohammad Jamir; Uddin, Md. Nazim; Adnan, Md.; Islam, Mohammad Nazmul; Sayeed, Mohammed Aktar; Emran, Talha Bin; Gandara, Jesus Simal; Ester Pagano 5; Rafandfaele Capasso 6Lepidagathis hyalina Nees is used locally in Ayurvedic medicine to treat coughs and cardiovascular diseases. This study explored its pharmacological potential through in vivo and in vitro approaches for the metabolites extracted (methanolic) from the stems of L. hyalina. A qualitative phytochemical analysis revealed the presence of numerous secondary metabolites. The methanol extract of L. hyalina stems (MELHS) showed a strong antioxidative activity in the 1,1-diphenyl-2- picrylhydrazyl (DPPH) and reducing power assays, and in the quantitative (phenolic and flavonoid) assay. Clot lysis and brine shrimp lethality bioassays were applied to investigate the thrombolytic and cytotoxic activities, respectively. MELHS exhibited an expressive percentage of clot lysis (33.98%) with a moderately toxic (115.11 g/mL) effect. The in vivo anxiolytic activity was studied by an elevated plus maze test, whereas the antidepressant activity was examined by a tail suspension test and forced swimming test. During the anxiolytic evaluation, MELHS exhibited a significant dosedependent reduction of anxiety, in which the 400 mg/kg dose of the extract showed 78.77 4.42% time spent in the open arm in the elevated plus maze test. In addition, MELHS demonstrated dosedependent and significant activities in the tail suspension test and forced swimming test, whereas the 400 mg/kg dose of the extract showed 87.67 6.40% and 83.33 6.39% inhibition of immobile time, respectively. Therefore, the current study suggests that L. hyalina could be a potential source of anti-oxidative, cytotoxic, thrombolytic, anxiolytic, and antidepressant agents. Further study is needed to determine the mechanism behind the bioactivities.