Department of Pharmacy
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Item Chemical, biological and protein-receptor binding profiling of Bauhinia scandens L. stems provide new insights into the management of pain, inflammation, pyrexia and thrombosis((http://creativecommons.org/licenses/by-nc-nd/4.0/)., 2021-09-08) Emon, Nazim Uddin; Rudra, Sajib; Alam, Safaet; Al Haidar, Ibrahim Khalil; Paul, Susmita; Richi, Fahmida Tasnim; Shahriar, Saimon; Sayeed, Mohammed Aktar; Tumpa, Nadia Islam; Ganguly, AmlanBauhinia scandens L. (Family, Fabaceae) is a medicinal plant used for conventional and societal medication in Ayurveda. The present study has been conducted to screen the chemical, pharmacological and biochemical potentiality of the methanol extracts of B. scandens stems (MEBS) along with its related fractions including carbon tetrachloride (CTBS), di-chloromethane (DMBS) and n-butanol (BTBS). UPLC-QTOF–MS has been implemented to analyze the chemical compounds of the methanol extracts of Bauhinia scandens stems. Additionally, antinociceptive and anti-inflammatory effects were performed by following the acetic acid-induced writhing test and formalin-mediated paw licking test in the mice model. The antipyretic investigation was performed by Brewer Yeast induced pyrexia method. The clot lysis method was implemented to screen the thrombolytic activity in human serum. Besides, the in silico study was performed for the five selected chemical compounds of Bauhinia scandens, found by UPLC-QTOF–MS By using Discover Studio 2020, UCSF Chimera, PyRx autodock vina and online tools. The MEBS and its fractions exhibited remarkable inhibition in dose dependant manner in the antinociceptive and antiinflammatory investigations. The antipyretic results of MEBS and DMBS were close to the standard drug indomethacin. Investigation of the thrombolytic effect of MEBS, CTBS, DMBS, and BTBS revealed notable clot-lytic potentials. Besides, the phenolic compounds of the plant extracts revealed strong binding affinity to the COX-1, COX-2, mPGES-1 and plasminogen activator enzymes. To recapitulate, based on the research work, Bauhinia scandens L. stem and its phytochemicals can be considered as prospective wellsprings for novel drug development and discovery by future researchers.Item Investigation of the Pharmacological Properties of Lepidagathis hyalina Nees through Experimental Approaches(https://www.mdpi.com/journal/life, 2021-02-25) Fahad, Fowzul Islam; Barua, Niloy; Islam, Md. Shafiqul; Sayem *, Al Jawad; Barua, Koushik; Uddin, Mohammad Jamir; Uddin, Md. Nazim; Adnan, Md.; Islam, Mohammad Nazmul; Sayeed, Mohammed Aktar; Emran, Talha Bin; Gandara, Jesus Simal; Ester Pagano 5; Rafandfaele Capasso 6Lepidagathis hyalina Nees is used locally in Ayurvedic medicine to treat coughs and cardiovascular diseases. This study explored its pharmacological potential through in vivo and in vitro approaches for the metabolites extracted (methanolic) from the stems of L. hyalina. A qualitative phytochemical analysis revealed the presence of numerous secondary metabolites. The methanol extract of L. hyalina stems (MELHS) showed a strong antioxidative activity in the 1,1-diphenyl-2- picrylhydrazyl (DPPH) and reducing power assays, and in the quantitative (phenolic and flavonoid) assay. Clot lysis and brine shrimp lethality bioassays were applied to investigate the thrombolytic and cytotoxic activities, respectively. MELHS exhibited an expressive percentage of clot lysis (33.98%) with a moderately toxic (115.11 g/mL) effect. The in vivo anxiolytic activity was studied by an elevated plus maze test, whereas the antidepressant activity was examined by a tail suspension test and forced swimming test. During the anxiolytic evaluation, MELHS exhibited a significant dosedependent reduction of anxiety, in which the 400 mg/kg dose of the extract showed 78.77 4.42% time spent in the open arm in the elevated plus maze test. In addition, MELHS demonstrated dosedependent and significant activities in the tail suspension test and forced swimming test, whereas the 400 mg/kg dose of the extract showed 87.67 6.40% and 83.33 6.39% inhibition of immobile time, respectively. Therefore, the current study suggests that L. hyalina could be a potential source of anti-oxidative, cytotoxic, thrombolytic, anxiolytic, and antidepressant agents. Further study is needed to determine the mechanism behind the bioactivities.Item Biological investigations of the methanol extract of Tetrastigma leucostaphylum (Dennst.) Alston ex Mabb. (Vitaceae): In vivo and in vitro approach(www.bsmiab.org, 2020-06-14) Rudra, Sajib; Sawon, Solaman Uddin; Emon, Nazim Uddin; Alam, Safaet; Tareq, Syed Mohammed; Islam, Mohammad Nazmul; Uddin, Md. Rokib; Md Sazid, Abdullah; Hasbe, Abu Nazer; Shakil, Mohammad; Sakib, Shahenur Alam; Sayeed, Mohammed AktarTetrastigma leucostaphylum (Family: Vitaceae) is popular for its medicinal value in Bangladeshi tribal communities. This study aims to investigate several pharmacological values of methanol extract of T. leucostaphylum (METL). In vivo analgesic and anti-inflammatory researches have been implemented by using acetic acid-induced writhing and formalin-induced paw licking test protocols in mice. Furthermore, in vitro thrombolytic and anthelmintic studies have been performed by following the blood clot lysis method and nematode mortality measurement method. In the in vivo study, METL did not minimize the acetic acid-induced writhes prominently but significantly attenuate both the peripheral and inflammatory pain in mice in a dose-dependent manner. In early and late phase, METL 400 (mg/kg, b.w; p.o) showed 39.63 % and 48.73 % paw licking inhibition. Again, METL (100 μL) reflected 56.62 % clot lysis in thrombolytic research. Besides, METL causes the death of nematodes in a dose-dependent manner. The bioassay of the methanol extract of T. leucostaphylum justified the analgesic, anti-inflammatory, thrombolytic and anthelmintic activities of the crude extract and finally suggests the test extract as a wellspring of anti-inflammatory, thrombolytic and anthelmintic agents as a crude drug source.Item Chemical Profiling, Pharmacological Insights and In Silico Studies of Methanol Seed Extract of Sterculia foetida(https://www.mdpi.com/journal/plants, 2021-06-03) Alam, Najmul; Banu, Naureen; Aziz, Arfin Ibn; Barua, Niloy; Ruman, Umme; Jahan, Israt; Jahan, Farhana; Denath, Susmita; Paul, Arkajyoti; Uddin, Nazim; Sayeed, Mohammed Aktar; Emran, Talha Bin; Gandara, Jesus SimalSterculia foetida, also known as jangli badam in Bangladesh, is a traditionally used plant that has pharmacological activities. A qualitative phytochemical analysis was performed to assess the metabolites in a methanolic extract of S. foetida seeds (MESF), and the cytotoxic, thrombolytic, anti-arthritics, analgesic, and antipyretic activities were examined using in vitro, in vivo, and in silico experiments. Quantitative studies were performed through gas chromatography-mass spectroscopy (GC-MS) analysis. The brine shrimp lethality bioassays and clot lysis were performed to investigate the cytotoxic and thrombolytic activities, respectively. The anti-arthritics activity was assessed using the albumin denaturation assay. Analgesic activity was determined using the acetic acidinduced writhing test and the formalin-induced paw-licking test. A molecular docking study was performed, and an online tool was used to perform ADME/T (absorption, distribution, metabolism, and excretion/toxicity) and PASS (Prediction of Activity Spectra for Substances). GC-MS analysis identified 29 compounds in MESF, consisting primarily of phenols, terpenoids, esters, and other organic compounds. MESF showed moderate cytotoxic activity against brine shrimp and significant thrombolytic and anti-arthritics activities compared with the relative standards. The extract also showed a dose-dependent and significant analgesic and antipyretic activities. Docking studies showed that 1-azuleneethanol, acetate returned the best scores for the tested enzymes. These findings suggested that MESF represents a potent source of thrombolytic, anti-arthritic, analgesic, antipyretic agents with moderate cytotoxic effects.Item IN VIVO AND IN VITRO EVALUATION OF PHARMACOLOGICAL ACTIVITIES OF Hedychium coccineum RHIZOMES EXTRACT(DOI: http://dx.doi.org/10.18006/2021.9(3).335.342, 2021-06-03) Nishi1, Sadia Islam; Barua1, Niloy2; Sayeed, Mohammed; Tareq1, Abu Montakim; Mina1, Sahnaj Begum; Emran3, Talha Bin; Dhama4, KuldeepThe study reports the in vivo antidiarrheal and in vitro anthelmintic, cytotoxic, and thrombolytic activity of methanol extract of Hedychium coccineum rhizomes (MEHC). The antidiarrheal activity was determined using Castor oil-induced diarrhea and Gastrointestinal motility test in mice at the doses of 200 and 400 mg/kg body weight, whereas an aquarium worm, Tubifex tubifex, was used to determine the anthelmintic activity. The cytotoxic and thrombolytic activity of MEHC was performed by Brine shrimp lethality bioassay and clot lysis method respectively. In antidiarrheal, castor oil-induced diarrhea and gastrointestinal motility exhibited a significant reduction in diarrhea and defecation and an extremely significant inhibition in intestinal motility and peristalsis index by 200 and 400 mg/kg of MEHC. The MEHC (5, 10, and 20 mg/mL) showed a significant dose-dependent manner paralysis time and times to death in multiple comparisons to the different levamisole concentrations (0.5, 0.8, and 1 mg/mL) at in vitro anthelmintic activity. The brine shrimp lethality bioassay exhibited a weak LC50 (681.95 μg/mL; R² = 0.951) while in thrombolytic a significant percentage of clot lysis (32.70%, P < 0.05) demonstrated. The findings demonstrate that H. coccineum rhizomes could be potential sources for biological activity.Item Evaluation of Antioxidant, Cytotoxic, Antibacterial Potential And Phytochemical Screening of Chloroform Extract of Phyllanthus Acidu(International Journal of Applied Biology and Pharmaceutical Technology, 2011-03) Mohammed, Aktar SayeedThe present study was carried out to evaluate the phytochemical properties, antimicrobial and cytotoxic as well as antioxidant activities of the chloroform extract.of Phyllanthus acidus fruit. Phytochemical screening showed confirmation of saponin, alkaloid, tannin and flavanoid. Antioxidant activity of PACF (Phyllanthus acidus chloroform extract) was assessed by using 1,1-diphenyl-2- picrylhydrazyl radical(DPPH),reducing power, cupric reducing antioxidant capacity and antioxidant activity increased in a concentration dependent manner. In DPPH radical scavenging assay IC50 value found 2745.86 µg mL-1 and compared to ascorbic acid with 13.37 µg mL-1. In brine shrimp lethality bioassay it showed good result with LC50 value 4.46 μg/ml. Antibacterial activity of plant extract was carried out using disc diffusion method with eleven pathogenic bacteria. This extract showed narrow spectrum activity aligned with Shigella dysenteriae, Escherichia coli, Staphylococcus aureus and Sarcina lutea at concentration of 500 µg/disc in comparison with standard kanamycin. The range of zone of inhibition of chloroform extract was 0.5 to 2.5 mm.Item Antidiarrheal, cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves(Published by www.bsmiab.org, 2020-01-19) Sayeed, Mohammed AktarABSTRACT: The study reports the in vivo antidiarrheal and in vitro cytotoxic and thrombolytic activities of methanolic extract of Hedychium coccineum leaves (MEHCL). The antidiarrheal activity was evaluated by castor oil-induced diarrhea, whereas the intestinal motility by charcoal marker. In addition, brine shrimp lethality bioassay and human blood clot lysis were used to evaluate the cytotoxic and thrombolytic activities, respectively. In antidiarrheal study, castor oil-induced diarrhea and gastrointestinal motility exhibited a significant dose dependent reduction in diarrhea and defecation and an extremely significant (P < 0.0001) inhibition in intestinal motility and peristalsis index by 200 and 400 mg/kg of MEHCL. The brine shrimp lethality bioassay revealed a considerable cytotoxic effect of MEHCL (LC50= 81.59 μg/mL; R² = 0.927) while in thrombolytic a significant percentage of clot lysis (17.36%, P < 0.01) demonstrated. The findings suggest that H. coccineum leaves could be potential sources for biological activity.