In vitro antimicrobial, cytotoxicity, antioxidant and In vivo analgesic activities of methanol extract of Dipterocarpus turbinatus leaves

Abstract

The study aimed to investigate the antimicrobial, cytotoxic, antioxidant, and analgesic properties of a methanol extract of Dipterocarpus turbinatus leaves. The extract shows a moderate antimicrobial effect against the tested organisms at different concentrations (10-19 mm of zone of inhibition). The percentage of mortality was observed to increase as the dose increased. The LC50 was found to be 408.50 μg/mL which proved the plant to be a good source of novel drugs with less toxicity. It exhibits antioxidant effect revealed by increase in the percentage of scavenging assay observed with increase in concentration. The presence of reductants in MEDT was also revealed. The number of writhing which is a dose dependent shows highest number at a dose of 200 mg/kg body weight i.e., 32.83±1.25 and 28.16±1.89, 15.5±2 for 400 mg/kg of MEDT and 10 mg/kg of Diclofenac-Na respectively. At a dose 0f 200 mg/kg of MEDT, 28.51±0.560 seconds were spent licking the paw while at a dose of 400 mg/kg of MEDT, 23.60±0.545 seconds were spent licking the paw.