IIUC Journal of Science and Engineering

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Author: search.filters.author.Ali, Md. Sekendar

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    Bacterial ghost: A novel strategy for medicinal applications
    (Center for Research and Publication (CRP), 2024-12) Ali, Md. Sekendar
    Bacterial ghost (BG) is an empty cell envelope of bacteria devoid of chromosomal and plasmid DNA and cytoplasmic content. However, it maintains the cellular morphology with native surface characteristics and bio adhesive properties. The widely used method to prepare BG is the protein E-mediated lysis method, which forms transmembrane tunnels through the inner and outer membranes of bacteria. There are also chemical and mechanical methods that lead to the formation of bacterial ghosts without damaging the necessary components and structure of bacteria. Alongside using NaOH, SDS, H2O2, tween 80, β-propiolactone, and 60% ethanol, hydrostatic pressure is also used to expel the cytoplasmic content resulting in pure envelop ghost. Recently developed holin-mediated inactivation, lysozyme, and protein kinase K are also used to kill bacteria, leaving them as BG. It is used in vaccines, immunotherapy of cancer, and a widely accepted delivery system of drugs, antigens, and other active components. The other efficient use of bacterial ghosts is to stimulate humoral and cell-mediated immunity through antigen-presenting cells. Taken together, BG represents a novel and emerging method for developing potential disease prevention and public health.
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    In vitro antimicrobial and antiarthritic effects of methanolic extract of Zanthoxylum rhetsa leaves
    (CRP, International Islamic University Chittagong, 2023-12) Sayeed, Mohammed Abu; Ali, Md. Sekendar; Chowdhuy, U Swe Sing; Islam, Ameerul; Ruzzama, Naiya; Muzammil Ahmad; Azad, Afrina
    The antimicrobial study of Zanthoxylum rhetsa leaves methanol extract was evaluated by the disc diffusion method against some pathogenic organisms. It exhibits the activity against almost all the test bacterial and fungal species with the zone of inhibition from mild to moderate in different concentrations- 100, 300 and 500 μg/disc where microbial species exhibits from minimum 10 to maximum 27 mm zone of inhibition. The Antiarthritic test have also done by using six concentrations for UV absorbance of test solution, product control solution, test control solution and percentage of inhibition of methanolic extract of Z. rhetsa with comparison of standard Diclofenac sodium in different concentrations like 31.75 μg/mL, 62.5 μg/mL, 125 μg/mL, 250 μg/mL, 500 μg/mL and 1000 μg/mL where different concentrations showed an increase in all the parameters that was significant from test, control and standard groups. The present study indicates that, the extract has both antibacterial and antifungal activity from mild to moderately in different concentrations and has significantly antiarthritic activities.