Mohammed, Aktar Sayeed2021-09-202021-09-202011-03www.ijabpt.com0976-4550http://dspace.iiuc.ac.bd:8080/xmlui/handle/123456789/2809The present study was carried out to evaluate the phytochemical properties, antimicrobial and cytotoxic as well as antioxidant activities of the chloroform extract.of Phyllanthus acidus fruit. Phytochemical screening showed confirmation of saponin, alkaloid, tannin and flavanoid. Antioxidant activity of PACF (Phyllanthus acidus chloroform extract) was assessed by using 1,1-diphenyl-2- picrylhydrazyl radical(DPPH),reducing power, cupric reducing antioxidant capacity and antioxidant activity increased in a concentration dependent manner. In DPPH radical scavenging assay IC50 value found 2745.86 µg mL-1 and compared to ascorbic acid with 13.37 µg mL-1. In brine shrimp lethality bioassay it showed good result with LC50 value 4.46 μg/ml. Antibacterial activity of plant extract was carried out using disc diffusion method with eleven pathogenic bacteria. This extract showed narrow spectrum activity aligned with Shigella dysenteriae, Escherichia coli, Staphylococcus aureus and Sarcina lutea at concentration of 500 µg/disc in comparison with standard kanamycin. The range of zone of inhibition of chloroform extract was 0.5 to 2.5 mm.enPhyllanthus AcidusAntioxidentCytotoxicityPhytochemical screeningArticle